DNA Replication: Once, and only once

DNA Replication: Once, and only once

The preparation for DNA replication initiation is tightly linked to cell-cycle progression, ensuring that replication occurs only once per cycle. The time is ripe for a molecular dissection of the links between the two processes.

Limiting DNA replication to once and only once.

In Escherichia coli cells, the origin of chromosomal replication is temporarily inactivated after initiation has occurred. Origin sequestration is the first line of defence against over-initiation, providing a time window during which the initiation potential can be reduced by: (i) titration of DnaA proteins to newly replicated chromosomal elements; (ii) regulation of the activity of the DnaA i...

Once and only once.

V enous thrombosis is a fairly common disorder that manifests mainly as deep vein thrombosis of the leg or pulmonary embolism. It affects 1 in 1000 individuals annually and has a substantial mortality rate. 1 Its causes are both genetic (prothrombotic abnormalities sometimes referred to as a group as thrombophilia) and acquired. 2 Among the latter are cancer, female hormone use, and all forms o...

Once and only once upon a time: specifying and regulating origins of DNA replication in eukaryotic cells.

Simultaneous Events and the "Once-Only" Effect

Some events recur, and some happen only once. Galton refers to the latter as “once-only" events [1]. In a first-order logic of events that makes a type-token distinction, the possibility of concurrent occurrences of the same event renders the characterization of the intuitive once-onliness not very intuitive. In particular, the paradigmatic case of the nth occurrence of a recurring event is sho...